The PRP was prepared from 10 ml of autologous blood drawn into a tube with 1.5 ml of acid citrate dextrose (an anticoagulant). The sample was centrifuged at 400 xg for 10 minutes and separated to yield 1.5 ml of PRP. Researchers added 1 ml of 3% calcium chloride to activate the platelets prior to injection. Injections were administered at 10 different locations spaced about 1.5 cm apart.
Injections or topical PRP was applied after the use of fractional ablative carbon dioxide laser. Ablative laser treatments injure the skin with microscopic thermal channels. As the skin heals, the bed of an atrophic scar is raised through re-epithelialization and collagen remodeling. PRP was shown to benefit the healing process by significantly reducing scar depth compared to placebo, with fewer side effects, and shortened downtime. A third party dermatologist evaluated before and after photos, assessing skin smoothness on a 4-point scale. Scar depth was measured with optical coherence tomography.³