An anticoagulant is necessary in the preparation of PRP. If no anticoagulant is used, a blood clot will form and the resulting serum will have platelet concentrations no higher than whole blood. Thus blood is collected in the presence of an anticoagulant, which serves to keep platelets from activating during the mechanical force of centrifugation. The Emcyte Pure PRP kit utilizes 10 ml of sodium citrate (SC) per 50 ml of whole blood, while the Harvest Clear PRP system relies on 6 ml of anticoagulant citrate dextrose solution A (ACD-A).
Researchers in Brazil (2016) investigated the influence of various anticoagulants on PRP platelet recovery rates.² The study design compared the effects of various anticoagulants, including SC, ethylenediaminetetraacetic acid (EDTA), and ACD-A. SC, utilized in Emcyte kits, produced PRP with the highest platelet recovery rate at 81.21% followed by EDTA and then ACD-A. Platelet recovery from ACD-A, as found in Harvest kits, were 45.71% almost half that of SC.
Platelet recovery rates dictate the potency of the final PRP serum, which directly affects patient outcomes. Results from this study suggest that differences in platelet recovery rate between the EmCyte PurePRP kit and the Harvest Clear system could be influenced by anticoagulant choice.